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The flow injection analysis (FIA) supplements CE 380. It uses the photometer in CE 380 as a detector to detect the reaction product glucose.
The multi-channel pump permanently conveys three liquid flows into the FIA. The dissolved reaction products from CE 380 and an indicator reagent are first mixed in one chamber. The mixture then flows through a helical reaction loop. The conduction of the flow in the reaction loop enables an even distribution of all substances. Another indicator reagent is added in a second mixing chamber. After flowing through another reaction loop, the mixture enters the flow cell. There the light intensity is continuously measured with the photometer to determine the glucose concentration. To trigger the discolouration for the photometric measurement, a defined amount of the enzyme glucose oxidase (GOD) is injected through an injection valve. The indicator reagents and the enzyme GOD are not included in the scope of delivery.
CE 380.01 enables more measurements during the experiment than a manual analysis. In addition, the reproducibility is improved and it is no longer necessary to mix each individual sample.
The well-structured instructional material sets out the fundamentals and provides a step-by-step guide through the experiments.
Learning Objectives / Experiments
- using the flow injection analysis (FIA)
- determining the concentration
- determining the yield for CE 380